Local Lymph Node Assay (LLNA)
 Printable Version

Outline of the Assay:

 
Determine optimal vehicle for test article (solubility testing)
Conduct Dermal Irritation Pre-Screen to determine test concentrations
Dose ears once daily for 3 consecutive days
On Day 6, (2 day rest period) injection of mice with BrdU
Sacrifice and isolation of the auricular lymph nodes
Individual Animal Analysis ("Pooled Nodes" protocol also available)
Processing/staining of lymph node cells (LNC)
Flow cytometric measurement of proliferation of LNC
Determination of the Stimulation Index (S.I.) for each group
Calculate, if possible, the EC3 (Minimal Sensitizing Concentration; where SI=3), the OECD and EPA criteria for positive sensitizers

 

MB Research Laboratories
Spinnerstown, PA  18968
voice:  (215) 536-4110
fax:  (215) 536-1816

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